3'-acetoxime-2'-deoxy nucleoside
Advantages of 3'-acetoxime-2'-deoxy nucleosides!
This novel nucleoside building block offers excellent advantages compared to conventional reagents with protective groups, as outlined below. 1. Extremely mild deprotection conditions - Conventional deprotection: In standard oligonucleotide synthesis, harsh conditions such as treatment with concentrated ammonia or high temperatures were required to cleave the oligomer from the support and remove protective groups (especially acyl protecting groups like benzoyl or isobutyryl). These conditions could damage unstable sequences, such as modified nucleosides or long-chain oligonucleotides. - Acetoxime protecting group: The 3'-acetoxime protecting group can be rapidly removed under extremely mild conditions, such as treatment at room temperature with water or weakly basic buffers (e.g., triethylammonium carbonate at pH 7-8). This dramatically improves purity and yield in the synthesis of complex probes and drugs, particularly those containing molecules sensitive to heat or bases (e.g., fluorescent dyes, thiolate modifications). 2. Reduction of synthesis cycle time Mild deprotection conditions shorten the overall time of the synthesis process.
- Company：サパラ
- Price：Other
